Mohamed Ghoneim

Over the course of my career, I developed an expertise in single-molecule biophysics of nucleic acid – protein interactions and biomolecular conformational dynamics. I obtained my Doctor of Philosophy in Biophysics from the University of Illinois at Urbana-Champaign, USA, where I used single-molecule multi-color total-internal fluorescence microscopy (TIRFM) to undertake direct real-time investigation of the correlation between the conformational dynamics of a DNA repair protein and binding to damage-containing nucleic acid. As a postdoctoral fellow in the Imperial College London, UK , I investigated the mechanism of nucleosome remodeling by the eukaryotic protein complexes using single-molecule FRET (smFRET). As a research scientist in Saint Louis University, USA, I studied Mechanism of Rad51 filament formation by Rad52 and Rad55-Rad57 in homologous recombination using single-molecule correlative optical trapping – fluorescence microscopy system. I also investigated the orientation of different domains of human RPA on different ssDNA-containing structures using alternating light excitation (ALEX) smFRET. More recently, I started focusing, in the University of Kansas, on investigating the conformational dynamics and interactions of viral RNA using single-molecule techniques.